FAQ
Posted on December 10, 2019
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
Ca2+calciumcalbrytecell assayprobenecidcalbryte 520no probenecidcalcium mobilitycalcium indicatororganic anion transporter
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
Ca2+calciumcalbrytecell assayprobenecidcalbryte 520no probenecidcalcium mobilitycalcium indicatororganic anion transporter
Posted on June 26, 2019
What is the working mechanism of Ionomycin?
calciumcalcium indicatorGPCRcalcium fluxcalcium mobilityIonomycinionophore
What is the working mechanism of Ionomycin?
calciumcalcium indicatorGPCRcalcium fluxcalcium mobilityIonomycinionophore
Posted on June 13, 2019
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCR
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCR
Posted on June 13, 2019
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
NADCa2+calciumcell assayfluorimetriccalcium mobilitysecond messengerfluorescent plate reader
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
NADCa2+calciumcell assayfluorimetriccalcium mobilitysecond messengerfluorescent plate reader
Posted on May 20, 2019
Which fluorescent calcium indicators are compatible with GFP positive cells?
Ca2+calcium indicatorcalcium fluxcell assayGPCRCalbrytefluorescent calcium indicatorcalcium mobility
Which fluorescent calcium indicators are compatible with GFP positive cells?
Ca2+calcium indicatorcalcium fluxcell assayGPCRCalbrytefluorescent calcium indicatorcalcium mobility
Posted on April 1, 2019
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator
Posted on April 1, 2019
What is the concentration of calcium inside cells?
calciumCa2+calcium indicatorcell assaycalcium fluxcalcium mobilityfluorescent calcium indicator
What is the concentration of calcium inside cells?
calciumCa2+calcium indicatorcell assaycalcium fluxcalcium mobilityfluorescent calcium indicator
Posted on September 26, 2018
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
Ca2+calciumcalcium indicatorcalcium mobilitycalcium fluxGPCRcalbrytecalbryte 520fluo-4
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
Ca2+calciumcalcium indicatorcalcium mobilitycalcium fluxGPCRcalbrytecalbryte 520fluo-4
Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator