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AAT Bioquest

FAQ

Posted on December 10, 2019
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
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Posted on June 26, 2019
What is the working mechanism of Ionomycin?
calciumcalcium indicatorGPCRcalcium fluxcalcium mobilityIonomycinionophore


Posted on June 13, 2019
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
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Posted on June 13, 2019
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
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Posted on May 20, 2019
Which fluorescent calcium indicators are compatible with GFP positive cells?
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Posted on April 1, 2019
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
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Posted on April 1, 2019
What is the concentration of calcium inside cells?
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Posted on September 26, 2018
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
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Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator


Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathways?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator


Posted on April 9, 2018
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
GPCRcalciumcalcium fluxcalcium mobilitycalcium indicatorEndoplasmic Reticulum (ER)